| 1. | Expression and identification of sars coronavirus s1 protein in prokaryotic cell
1蛋白的原核表达与鉴定 |
| 2. | Expression of gst - snail fusion protein in prokaryotic cells and preparation of polyclonal antibody against snail
融合蛋白的原核表达及其多克隆抗体制备 |
| 3. | Expression and identification of truncated 56kda protein of orientia tsutsugamushi gilliam strain in prokaryotic cells
蛋白部分基因的原核表达及初步鉴定 |
| 4. | Conclusion : the target gene of the domain iv of the chain v 2 could be gained from keratinocytes and expressed in prokaryotic cells in high efficiency
结论： ln - 5 2链功能区基因可从皮肤组织中获得，并在原核细胞中得到高效表达。 |
| 5. | Objective : to construct the single - chain fv gene of human anti - hbsag and to analyse the expression of the constructed gene in eukaryotic cell and prokaryotic cell
目的构建人抗hbsag单链抗体基因，在真核细胞和原核细胞中进行表达，并对其表达产物的活性进行鉴定。 |
| 6. | Our study establish a stable base for further reseach of hng and human insulin mutant and provide a theoretics gist for expression of low molecular weight proteins in prokaryotic cells
为用融合表达方式表达小分子量多肤及规模化制备小分子蛋自奠定了初步的实验和理论基础。 |
| 7. | Although the thymosin has been successfully expressed in the prokaryotic cell system , its application is restricted because of its deficiency of modification after translation
尽管研究者已在原核细胞表达系统对胸腺肽的表达进行了研究，但由于原核表达系统缺乏翻译后的加工修饰等缺点，限制了其应用。 |
| 8. | Polymer his - tagged peac1 - gfp efficiently activated myosin mg - atpase activity , which indicated that peacl might take part in correlative living activities in vivo . moreover , this result provided experimental proof in vitro for fusing gfp to actin isoform directly to study the dynamics of microfilaments and its regulation in vivo . we prepared rabbit anti - pea actins polyclonal antibodies using peacl as antigen which being expressed and purified from prokaryotic cells , and the antibodies possessed better immunity activity to pea actins
通过肌动蛋白体外对dnase以及肌球蛋白atpase活性影响的研究，发现单体his - taggedpeac1 - gfp能显著抑制dnase活性，在肌动蛋白聚合条件下能有效激活肌球蛋白atpase活性，这一结果预示着peac1在体内可能参与相关的生命活动，为利用gfp直接与肌动蛋白异型体融合来研究体内微丝的动态变化及其调节提供了实验依据。 |
| 9. | Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ) , strain bl21 ( de3 ) , to study their expression in prokaryotic cell . the gene was expressed under t7 promoter with a fusion protein partner of thx . tag and a 6x his . tag at its n - terminal . having been induced by iptg for 4 hours , the recombinant enzyme was examined in the cytoplasm by sds - page analysis
将大连蛇岛蝮蛇和长白山白眉蝮蛇毒类凝血酶基因分别克隆到大肠杆菌表达载体pet - 32 ( a ) +中，在t7启动子下表达出融合蛋白，融合部分为硫氧还蛋白，位于类凝血酶基因上游，并在其n端带6xhistag标签以利于表达产物的分离纯化，经热激转化至宿主菌bl21 ( de3 )中， iptg诱导斗小时后收获菌体。 |
| 10. | Human bone morphogenetic protein 3 is a member of tgf - b superfamily . lt can induce the differentiation of cartilage and bone tissue in mesenchymal cell . and is important to bone self - repairment and bone development during embryo morphogenesis . in addition , some other biological activities of hbmp - 3 have also been found . such as inducing development of embryo and stimulating differentiation of neural and blood cells . therefore , there is a great prospect in the use of hbmp - 3 . there is trace content of hbmp - 3 in human body . it has been expressed in the expression system of eukaryotes and prokaryotes respectively , but its application is restricted because of defects in the process and modification after translation in prokaryotic cells and higher costs and lower yields existed in eukaryotic expression system
人骨形成蛋白3 ( hbmp - 3 )属于tgf -超家族的一员，可以诱导间充质细胞分化为软骨和骨，在胚胎时期骨骼发育和骨再生修复中起着重要的作用，而且对胚胎发育过程中中胚层的诱导和分化、造血组织的发育以及神经系统的发育和修复等都起着重要作用，因而hbmp - 3有广阔的市场前景。它在人体内含量极微，尽管研究人员已经在原核细胞和真核细胞表达系统中分别进行了表达，但是由于原核表达系统缺乏翻译后的加工修饰，真核表达系统存在成本高、产量低等特点，限制了其在临床上的应用。 |
